ğReady-to-UseĞ M-PVA Magnetic Beads

M-PVA SAVx; Streptavidin-functionalized

Standard particle sizes:

M-PVA SAV1: 0.5 - 1 µm (article No. 227)

M-PVA SAV2: 1 - 3 µm (article No. 228)

Min. quantity delivered:

2 ml bead suspension

Concentration:

25mg/mlMagnetite: 50 - 60 %

Storage:

in PBS-Puffer pH 7.2; 0.1 % bovine serum albumine (for Stabilization) 0.05 % Sodium azide

Binding Capacity:

M-PVA SAV1
30 - 50 pmol biotinylated protein/mg
at least 400 pmol biotynylated oligonucleotide/mg

M- PVA SAV2
30 - 40 pmol biotynlated protein/mg
at least 300 pmol biotinylated oligonucleotide/mg
520 free biotin/mg

Properties: Streptavidin is immobilized covalently on the surface of superparamagnetic polyvinyl alcohol beads. Their high magnetite content permits the rapid separation of biotinylated target molecules. Because of thehydrophilic nature of the polyvinylalcohol matrix, unspecific binding properties are reduced to a minimum. These beads have a polydisperse size distribution.

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M-PVA OdTx; Oligo(dT)30-functionalized

Standard particle sizes:

M-PVA OdT1: 0.5 - 1 µm (article No. 230)

M-PVA OdT2: 1 - 3 µm (article No. 231)

Min. quantity delivered:

2 ml bead suspension

Concentration:

25 mg/ml

Magnetite:

50 - 60 %

Storage:

in TE-Puffer (10 mM Tris, 1 mM EDTA, pH 8.0); 0.05 % sodium azide

Binding capacity:

M-PVA OdT1
1 - 2 µg polyadenylated NA/mg

M-PVA OdT2
1 - 2 µg polyadenylated NA/mg

Properties: Oligo(dT) is immobilized on the surface of superparamagnetic polyvinyl alcohol beads. Their high magnetite content permits the rapid separation of polyadenylated target molecules. For optimal separation results, we recommend the use of a binding buffer with a 0.5 mol/l sodium chloride salt concentration. These beads have a polydisperse size distribution.