The Better Way of Magnetic Separation
To ease and to accelerate nucleic acid isolation based on magnetic particles and to offer the user a highly flexible and reliable automation, chemagen together with the Forschungszentrum Karlsruhe GmbH has developed a unique separation method with outstanding possibilities.
The separation of nucleic acids is achieved through their capturing by highly specific binding M-PVA Magnetic Beads that are thereafter attracted to metal rods, magnetised by an electromagnet. While these magnetised rods transfer the DNA bound to the particles through the different process solutions, the rod rotation, switched on after deactivation of the electromagnet, leads to efficient and homogenious resuspension of the particels during the preparation steps. This results in high yield purity DNA/RNA and success in the following downstream applications.
In the animation below you can see the separation process as implemented in the chemagic Magnetic Separation Module I.
In the first step the rods take up tips to protect them from contamination through sample material or resting particles. The next step shows the attraction of particles that are subsequently immersed into the sample solution in the third step. Here, while the electomagnet is switched off, they are rotated off the rods to bind the DNA. After the binding, the electomagnet is switched on again and the DNA bound to the particles is attracted to the rods. Step four shows the washing procedure that is repeated several times according to the protocol. The elution of the nucleic acids in step five takes place in the same way as the previous steps. During the rod rotation the DNA is eluted from the beads that are thereafter attracted to the magnetised rods again. In the last step the tips are automatically striped off the rods and the beads are thereby disposed.
In the bottom right corner of the animation you can switch between the different process steps or pause the animation using your left mouse button.
