Development of methods effective for detection and decrease of host-cell proteins from therapeutical antibodies
Targets of the project:
- Development of a new, very sensitive and selective method, applicable for high throughput applications, to quantify host cell proteins (HCP). Furthermore this method should facilitate the decrease of particular HCPs in a pool of HCPs even in the presence of high concentrations of active agents. During the several chromatography projects the decreasing rate should be detected quantitatively and with highest sensitivity.
- Identification of those HCP, which are difficult to remove in antibody-isolation processes.
- Solution for efficient decrease of these selected HCP in column chromatography based on their specific protein-chemical characteristics and accompanied with mp-MS-quant Analytic.
Conclusion: In case of success, bottle necks in downstream processing during the production of bio-pharmaceutical proteins will be abandoned and in addition the whole process will be much saver and will fulfill all quality standards. Furthermore the production activity will become more economical.
Cooperation partners:
- chemagen AG
- AK Feldhusen, IKT, RWTH Aachen
- AK Schlüter, Inst. for clin. Chemistry
- Agilent Technologies
- AK Frech, Hochschule Mannheim
- Boehringer-Ingelheim
- University Clinic Hamburg-Eppendorf (UE)
- Agilent Technologies
- Wita GmbH
