Nucleic Acid Isolation from Food Samples
The chemagic Food Basic Kit is designed for DNA extraction and purification of
genomic DNA from 200 mg food samples of mainly plant origin. Lysis of food material and binding conditions are optimized to obtain both high yield and high quality of the genomic DNA. The purified DNA is suitable for downstream applications such as PCR and GMO detection. To increase the sensitivity of detection the sample size is scalable for difficulte samples such as processed food. For preparing lysates from 2 g of food samples material the chemagic Food Extension Kit is available.
Fig. A: Genomic DNA purified from 200 mg food material using various kits.
Genomic DNA isolated from maize using
(1) chemagic Food Basic Kit
(2) competitor Kit (based on magnetic beads).
Genomic DNA isolated from rape using
(3) chemagic Food Basic Kit
(4) competitor Kit (based on magnetic beads)
Fig. B: PCR detection of food-specific products.
(1) rape chemagic Food Basic Kit
(2) rape competitor Kit (based on magnetic beads)
(3) rape negative control (4) size marker pUC/MspI (5) lecithin
chemagic Food Basic Kit
(6) lecithin competitor Kit ( based on magnetic beads) (7) lecithin negative control
(8) size marker pUC/MspI (9) maize chemagic Food Basic Kit
(10) maize competitor Kit (based on magnetic beads) (11) maize negative control.
